%0 Journal Article
%T The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic bone tissues
%A Janja Zupan
%A Radko Komadina
%A Janja Marc
%J Journal of Biomedical Science
%D 2012
%I BioMed Central
%R 10.1186/1423-0127-19-28
%X Human bone samples from 54 age and sex matched patients with OP or OA were collected during hip arthroplasty surgery. The expression of 25 genes encoding pro-inflammatory cytokines, their receptors, osteoclast specific genes and RANK/RANKL/OPG genes was measured using quantitative real-time PCR. Total hip, femoral neck and lumbar spine BMD and BTM in blood samples were measured. The comparison between OP and OA was assessed using Student's t-test or Mann-Whitney U test and correlations between gene expression, BMD and BTM were determined using nonparametric correlation.The results demonstrated a higher expression of interleukin (IL)-6 and IL-1¦Á in OP, and interferon (IFN)-¦Ã in OA (p < 0.0005). Negative correlations of total hip BMD with tumor necrosis factor-¦Á (TNF-¦Á) in OA and with RANKL/RANK in OP were found (p < 0.05). Significant correlations with BTM were shown for IL-1¦Á and IFN-¦Ã in OP (rho = 0.608 and -0.634) and for TNF-¦Á, IL-6 and transforming growth factor-¦Â1 (TGF-¦Â1) in OA (rho = 0.591, -0.521 and 0.636). Results showed OP specific negative correlations (IFN-¦Ã with ITGB3, IFN-¦Â1 with CTSK, tartrate resistant acid phosphatase (TRAP), CALCR, RANK, RANKL, IL-1¦Á with CTSK, OPG, IL-17A with CALCR) and positive (TGF-¦Â1 with CTSK, TRAP, RANK), and OA specific negative (IL-1¦Á with osteoclast associated immunoglobulin-like receptor (OSCAR), TNF-¦Á with RANK, RANKL, OPG) and positive (IL-6 with RANK, RANKL, OPG) correlations.Our results demonstrate that the relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human OP and OA bone and could present an important factor for characteristics of OP and OA bone phenotypes.Osteoclasts are influenced by a variety of pro-inflammatory osteoclastogenic and anti-osteoclastogenic cytokines that can either stimulate or suppress their activity [1]. This regulation of osteoclasts becomes particularly important in the pathological activation of the immune system, when pro-inflammatory
%K Interleukins
%K Interferons
%K TNF-¦Á
%K TGF-¦Â1
%K ¦Â3 integrin
%K Cathepsin K
%K OSCAR
%U http://www.jbiomedsci.com/content/19/1/28