%0 Journal Article
%T Screening of alkalophilic thermophilic protease isolated from Bacillus RV.B2.90 for Industrial applications
%A Vijayalakshmi S
%A Venkatkumar S
%A Thankamani V
%J Research in Biotechnology
%D 2011
%I 
%X Protease enzyme from Bacillus RV.B2.90 was purified, fractionated and tested forvarious applications. This enzyme could degrade the natural proteins like coagulated eggand blood clot. Blood and natural pigment stain (carrot, beetroot, green leaves, coffee andtea) were removed by this enzyme easily. It exhibited good compatibility with thecommercial detergents such as Surf Excel, Tide, Ujala, Ariel and Rin. The keratinolyticactivity of the enzyme was evidenced by the complete degradation of feathers. X-ray filmstreated with the enzyme showed release of protein from the gelatin coating, a pre requisitefor recovery of silver. Also showed better stability with surfactants and solvents, which willbe advantageous as detergent formulations containing chelating agents like EDTA and inpeptide synthesis. The partially purified enzyme showed direct hydrolysis of various naturalproteins (BSA, casein and azocasein) applied to clean glass slides followed by denaturationby boiling. Bacillus RV.B2.90 immobilization with 3% alginate showed maximumproduction (2002 U/ml). In continuous production, the beads were stable over 24 hrs X 9cycles. RV.B2.90 protease highly thermostable and alkalophilic with a variety of activities,has great potential for application in a wide range of industry.
%K Bacillus RV.B2.90
%K alkalophilic
%K thermophilic
%K Protease
%K Industrial applications
%U http://www.researchinbiotechnology.com/view/article/2/3/4