%0 Journal Article
%T An invitro antimicrobial activity and Bioactivities of Protein Isolated from Marine Sponge ¨C Callyspongia sp.
%A Dhanalakshmi. J
%A Biwott Felix Kipkirui
%A Selvi. S
%J Research in Pharmacy
%D 2012
%I GKS Publisher
%X The marine sponge Callyspongia sp, collected from Mandapam coast, Tamilnadu, wasstudied for antibacterial and antifungal activity of proteins. Sponge species were identifiedbased on spicules morphology. Chloroform and aqueous extracts of Callyspongia sp yieldeda total amount of 4.8g and 5.98g from 500g of sponge respectively. Crude protein obtainedfrom marine sponge aqueous extract was 2.1 mg/ml and that of chloroform extract was 0.9mg/ml. The antimicrobial activity of chloroform extract and aqueous extract showed clearinhibition zone against Pseudomonas sp. Vibrio cholerae, Aspergillus niger and Candidaalbicans. Both the extracts exhibited hemolytic activity which was estimated as 10.1ht/ml forchloroform extract and 8.6ht/ml for aqueous extract. The partial purification of protein wascarried out using DEAE cellulose. On SDS-PAGE the crude protein yielded three welldefined bands at 109.9, 28.2, 12.4 KDa respectively by both the extracts.
%K Callyspongia sp.
%K protein purification
%K Candida albicans chloroform extract
%K and Aspergillus niger.
%U http://www.researchinpharmacy.com/view/article/4/1/5