%0 Journal Article
%T Response gene to complement-32 enhances metastatic phenotype by mediating transforming growth factor beta-induced epithelial-mesenchymal transition in human pancreatic cancer cell line BxPC-3
%A Liang Zhu
%A Hua Qin
%A Pei-Yuan Li
%A Sheng-Nan Xu
%A Hui-Fang Pang
%A Hui-Zhen Zhao
%A De-Min Li
%A Qiu Zhao
%J Journal of Experimental & Clinical Cancer Research
%D 2012
%I BioMed Central
%R 10.1186/1756-9966-31-29
%X Immunohistochemical staining of RGC-32 and E-cadherin was performed on specimens from 42 patients with pancreatic cancer, 12 with chronic pancreatitis and 8 with normal pancreas. To evaluate the role of RGC-32 in TGF-¦Â-induced EMT in pancreatic cancer cells, BxPC-3 cells were treated with TGF-¦Â1, and RGC-32 siRNA silencing and gene overexpression were performed as well. The mRNA expression and protein expression of RGC-32 and EMT markers such E-cadherin and vimentin were determined by quantitative reverse transcription-PCR (qRT-PCR) and western blot respectively. Finally, migration ability of BxPC-3 cells treated with TGF-¦Â and RGC-32 siRNA transfection was examined by transwell cell migration assay.We found stronger expression of RGC-32 and higher abnormal expression rate of E-cadherin in pancreatic cancer tissues than those in chronic pancreatitis tissues and normal pancreatic tissues. Immunohistochemical analysis revealed that both RGC-32 positive expression and E-cadherin abnormal expression in pancreatic cancer were correlated with lymph node metastasis and TNM staging. In addition, a significant and positive correlation was found between positive expression of RGC-32 and abnormal expression of E-cadherin. Furthermore, in vitro, we found sustained TGF-¦Â stimuli induced EMT and up-regulated RGC-32 expression in BxPC-3 cells. By means of siRNA silencing and gene overexpression, we further demonstrated that RGC-32 mediated TGF-¦Â-induced EMT and migration in BxPC-3 cells.The results above indicated that RGC-32 might be a novel metastasis promoting gene in pancreatic cancer and it enhances metastatic phenotype by mediating TGF-¦Â-induced EMT in human pancreatic cancer cell line BxPC-3.Pancreatic cancer is one of the most lethal human cancers due to its high metastatic potential, late manifestation of symptoms and strong chemoresistance [1]. Although more and more therapies including surgical resection, chemotherapy and radiotherapy have been used in recent years, pat
%K Response gene to complement-32
%K Pancreatic cancer
%K Transforming growth factor -¦Â
%K Epithelial-mesenchymal transition
%K Migration
%U http://www.jeccr.com/content/31/1/29