%0 Journal Article
%T BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells
%A Jing Zhu
%A Li Sun
%A Sensen Lin
%A Renping Zhao
%A Liqiang Zhou
%A Dongdong Fang
%A Liang Chen
%A Jin Liu
%A Wenting Shi
%A Luyong Zhang
%A Shengtao Yuan
%J Journal of Experimental & Clinical Cancer Research
%D 2012
%I BioMed Central
%R 10.1186/1756-9966-31-31
%X In this study, we examined the role of BLyS in the migration of human breast cancer cells by transwell assay. We also explored whether BLyS and its receptors expressed in human breast cancer cell lines by immunofluorescence and Western Blotting. Then we detected the expression level of BLyS in both normoxic and hypoxic conditions by real time-PCR and Western Blotting. Pathways involved were confirmed by Western Blotting, immunofluorescence, transwell assay and luciferase assay.According to our study, the expression level of BlyS was increased in human breast cancer cell lines in hypoxic conditions. Up-regulation of this protein led to activation and nuclear translocation of NF-kappa B p65. We also found that the number of migrated cells was increased in the presence of BLyS and inhibition of phosphorylation of Akt attenuated the enhanced migratory response.It suggested that better understanding of BLyS, an immunopotentiator, may offer a potential therapeutic target for the treatment of human breast cancers. In addition, BLyS promoted breast cancer cells migration, underscoring the necessity of appropriate applications of immunopotentiators to cancer treatment.B Lymphocyte Stimulator (BLyS), a key member of the tumor necrosis factor superfamily, binds to three receptors: B-cell maturation antigen (BCMA), transmembrane activator and CAML interactor (TACI), and B cell-activating factor receptor (BAFF-R). BLyS promotes survival of splenic immature transitional and mature B cells [1]. Over-expression of BLyS has been associated with multiple myeloma (MM) [2], Systemic lupus erythematosus (SLE) [3] and B cell lymphoma [4]. It has also been reported that this ligand/receptor dyad plays a critical role in the growth and survival of malignant plasma cells and B cells [5]. Recent studies in ductal breast cancer patients have suggested a role of BLyS in the development of breast cancer. But its molecular mechanisms remain to be elucidated [6].Hypoxia plays a significant role i
%K Hypoxia
%K BLyS
%K Cell migration
%U http://www.jeccr.com/content/31/1/31