%0 Journal Article
%T Adipose tissue biglycan as a potential anti-inflammatory target of sodium salicylate in mice fed a high fat diet
%A Venkatta J Adapala
%A Meliza Ward
%A Kolapo Ajuwon
%J Journal of Inflammation
%D 2012
%I BioMed Central
%R 10.1186/1476-9255-9-15
%X C57BL6 mice were fed either a control (10% fat calories) or a high fat diet (HFD) (60% fat calories) for 8 weeks. Adipose tissue was analyzed for the expression of biglycan, IL-6 and TNF¦Á. Biglycan knockout or wild type were also fed a high fat diet for 8 weeks and the expression of inflammatory genes in the mesenteric adipose tissue was examined. To test anti-inflammatory treatment on biglycan expression, a group of mice were fed either the low fat or high fat diet for eight weeks supplemented with either saline or sodium salicylate @ 25mg/100ml in their drinking water.Mice on HFD had an increase in ECM genes (BGN and COL1A1), inflammatory genes (IL-6 and TNF¦Á) in both the subcutaneous and epididymal depots. However, correlation analysis only shows a positive correlation between biglycan, IL-6 and TNF¦Á expression. In addition, lower expression of IL-6 and CD68 was found in the mesenteric adipose tissue of biglycan knockout mice compared to the wild type. Sodium salicylate treatment reduced subcutaneous adipose tissue expression of BGN, COL1A1, and COL6A1 and a concurrent downregulation of TNF¦Á and IL-6 and TLR4 expression. Salicylate also lowered the serum TGF¦Â1 levels.Biglycan expression correlates with adipose tissue inflammation, especially in the subcutaneous depot compared to the epididymal depot. This is supported by the greater effect of sodium salicylate in attenuating both inflammatory and ECM gene expression the subcutaneous adipose depot compared to the epididymal depot. These results show that inflammatory state may explain the induction of biglycan, and perhaps, other ECM genes in adipose tissue.
%U http://www.journal-inflammation.com/content/9/1/15/abstract