%0 Journal Article
%T Plasmid profiling of multidrug resistant Escherichia coli strains isolated from urinary tract infection patients
%A Sabin Khadgi
%A Uddhav Timilsina
%A Basudha Shrestha
%J International Journal of Applied Sciences and Biotechnology
%D 2013
%I SEM-Biotech Publishing
%R 10.3126/ijasbt.v1i1.7918
%X Introduction- Urinary tract infection is a common community-acquired bacterial disease. Escherichia coli is reported to be the major cause of urinary tract infection. Aim & Objective- The study was conducted with the aim of determining the antibiotic resistance pattern and plasmid profile of multidrug resistant Escherichia coli isolated from Urinary Tract Infection patients. Materials and Method- Antibiotic susceptibility tests were performed against E. coli following the protocol for the Kirby-Bauer disc diffusion method. Plasmid DNA was isolated following the protocol of Kado and Liu. Results- Multidrug resistant isolates exhibited high resistance to drugs like Amoxicillin, Cefixime, Ciprofloxacin, Cotrimethoxazole, Norfloxacin and Ofloxacin. The plasmid profiling showed that all, except one, isolate contained at least one plasmid. A band of approximately 23 kb was seen in most of the isolates.
%K urinary tract infection
%K antibiotic resistance
%K antibiotic susceptibility
%K escherichia coli
%K plasmid profile
%U http://ijasbt.org/vol_1/khadgi_et.al._IJASBT_1(1).pdf