%0 Journal Article
%T Recombinant vectors construction for cellobiohydrolase encoding gene constitutive expression
%A Leontina GURGU
%J Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology
%D 2012
%I Galati University Press
%X Cellobiohydrolases (EC 3.2.1.91) are important exo enzymes involved in cellulose hydrolysis alongside endoglucanases (EC 3.2.1.4) and ¦Â-glucosidases (EC 3.2.1.21). Heterologous cellobiohydrolase gene expression under constitutive promoter control using Saccharomyces cerevisiae as host system is of great importance for a successful SSF process. From this point of view, the main objective of the work was to use Yeplac181 expression vector as a recipient for cellobiohdrolase - cbhB encoding gene expression under the control of the actin promoter, in Saccharomyces cerevisiae. Two hybridvectors, YEplac-Actp and YEplac-Actp-CbhB, were generated usingEscherichia coli XLI Blue for the cloning experiments. Constitutive cbhB gene expression was checked by proteine gel electrophoresis (SDS-PAGE) after insertion of these constructs into Saccharomyces cerevisiae.
%K cellobiohydrolase I
%K constitutive promoter
%K gene expression
%U http://www.ann.ugal.ro/tpa/Anale%202012/vol2/4_LGurgu.pdf