%0 Journal Article
%T Encapsulation of Alpha-1 antitrypsin in PLGA nanoparticles: In Vitro characterization as an effective aerosol formulation in pulmonary diseases
%A nazanin pirooznia
%A sadegh hasannia
%A abas Sahebghadam Lotfi
%A mostafa ghanei
%J Journal of Nanobiotechnology
%D 2012
%I BioMed Central
%R 10.1186/1477-3155-10-20
%X Nonaqueous and double emulsion techniques were applied for the synthesis of nanoparticles. Nanoparticles were characterized in terms of surface morphology, size distribution, powder X-ray diffraction (XRD), encapsulation efficiency, in vitro drug release, FTIR spectroscopy and differential scanning calorimetry (DSC). To evaluate the nanoparticles cytotoxicity, cell cytotoxicity test was carried out on the Cor L105 human epithelial lung cancer cell line.Nanoparticles were spherical with an average size in the range of 100 nm to 1¦Ì. The encapsulation efficiency was found to be higher when the double emulsion technique was applied. XRD and DSC results indicated that ¦Á1AT encapsulated in the nanoparticles existed in an amorphous or disordered-crystalline status in the polymer matrix. The lactic acid to glycolic acid ratio affects the release profile of ¦Á1AT. Hence, PLGA with a 50:50 ratios exhibited the ability to release %60 of the drug within 8, but the polymer with a ratio of 75:25 had a continuous and longer release profile. Cytotoxicity studies showed that nanoparticles do not affect cell growth and were not toxic to cells.In summary, ¦Á1AT-loaded nanoparticles may be considered as a novel formulation for efficient treatment of many pulmonary diseases.
%K Cytotoxicity
%K DSC (differential scanning calorimetry)
%K FTIR
%K Nanoparticle
%K Sustained drug release
%K XRD (x-ray diffraction)
%U http://www.jnanobiotechnology.com/content/10/1/20/abstract