%0 Journal Article
%T Proteomic Analysis on Acetate Metabolism in Citrobacter sp. BL-4
%A Young-Man Kim
%A Sung-Eun Lee
%A Byeoung-Soo Park
%A Mi-Kyung Son
%A Young-Mi Jung
%A Seung-Ok Yang
%A Hyung-Kyoon Choi
%A Sung-Ho Hur
%A Jong Hwa Yum
%J International Journal of Biological Sciences
%D 2012
%I Ivyspring International Publisher
%X Mass production of glucosamine (GlcN) using microbial cells is a worthy approach to increase added values and keep safety problems in GlcN production process. Prior to set up a microbial cellular platform, this study was to assess acetate metabolism in Citrobacter sp. BL-4 (BL-4) which has produced a polyglucosamine PGB-2. The LC-MS analysis was conducted after protein separation on the 1D-PAGE to accomplish the purpose of this study. 280 proteins were totally identified and 188 proteins were separated as acetate-related proteins in BL-4. Acetate was converted to acetyl-CoA by acetyl-CoA synthetase up-regulated in the acetate medium. The glyoxylate bypass in the acetate medium was up-regulated with over-expression of isocitrate lyases and 2D-PAGE confirmed this differential expression. Using 1H-NMR analysis, the product of isocitrate lyases, succinate, increased about 15 times in the acetate medium. During acetate metabolism proteins involved in the lipid metabolism and hexosamine biosynthesis were over-expressed in the acetate medium, while proteins involved in TCA cycle, pentose phosphate cycle and purine metabolism were down-regulated. Taken together, the results from the proteomic analysis can be applied to improve GlcN production and to develop metabolic engineering in BL-4.
%U http://www.biolsci.org/v08p0066.htm