%0 Journal Article
%T Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1
%A Yunxue Guo
%A Yaosheng Chen
%A Yun Zhang
%A Yue Zhang
%A Luxi Chen
%A Delin Mo
%J International Journal of Biological Sciences
%D 2012
%I Ivyspring International Publisher
%X Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein ¦Á (C/EBP¦Á), and peroxisome proliferator-activated receptor ¦Ã2 (PPAR¦Ã2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.
%U http://www.biolsci.org/v08p1408.htm