%0 Journal Article
%T Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
%A Guido Dentesano
%A Marco Straccia
%A Aroa Ejarque-Ortiz
%A Josep M Tuselll
%A Joan Serratosa
%A Josep Saura
%A Carme Sol¨¤
%J Journal of Neuroinflammation
%D 2012
%I BioMed Central
%R 10.1186/1742-2094-9-165
%X Murine primary microglial cultures, mixed glial cultures from wild-type and CCAAT/enhancer binding protein ¦Â (C/EBP¦Â)-deficient mice, and the BV2 murine cell line overexpressing C/EBP¦Â were used to study the involvement of C/EBP¦Â transcription factor in the regulation of CD200R1 expression in response to a proinflammatory stimulus (lipopolysaccharide (LPS)). Binding of C/EBP¦Â to the CD200R1 promoter was determined by quantitative chromatin immunoprecipitation (qChIP). The involvement of histone deacetylase 1 in the control of CD200R1 expression by C/EBP¦Â was also determined by co-immunoprecipitation and qChIP.LPS treatment induced a decrease in CD200R1 mRNA and protein expression in microglial cells, an effect that was not observed in the absence of C/EBP¦Â. C/EBP¦Â overexpression in BV2 cells resulted in a decrease in basal CD200R1 mRNA and protein expression. In addition, C/EBP¦Â binding to the CD200R1 promoter was observed in LPS-treated but not in control glial cells, and also in control BV2 cells overexpressing C/EBP¦Â. Finally, we observed that histone deacetylase 1 co-immunoprecipitated with C/EBP¦Â and showed binding to a C/EBP¦Â consensus sequence of the CD200R1 promoter in LPS-treated glial cells. Moreover, histone deacetylase 1 inhibitors reversed the decrease in CD200R1 expression induced by LPS treatment.CD200R1 expression decreases in microglial cells in the presence of a pro-inflammatory stimulus, an effect that is regulated, at least in part, by C/EBP¦Â. Histone deacetylase 1 may mediate C/EBP¦Â inhibition of CD200R1 expression, through a direct effect on C/EBP¦Â transcriptional activity and/or on chromatin structure.
%K Neuroinflammation
%K Reactive microglia
%K CD200R1
%K C/EBP¦Â
%K Neuron-microglia communication
%K In vitro
%U http://www.jneuroinflammation.com/content/9/1/165/abstract