%0 Journal Article
%T (£¿)-Epigallocatechin gallate inhibits endotoxin-induced expression of inflammatory cytokines in human cerebral microvascular endothelial cells
%A Jieliang Li
%A Li Ye
%A Xu Wang
%A Jinping Liu
%A Yizhong Wang
%A Yu Zhou
%A Wenzhe Ho
%J Journal of Neuroinflammation
%D 2012
%I BioMed Central
%R 10.1186/1742-2094-9-161
%X The expression of TNF-¦Á, IL-1¦Â and monocyte chemotactic protein-1 (MCP-1/CCL2) was determined by quantitative real time PCR (qRT-PCR) and ELISA. Intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule (VCAM) in hCMECs were examined by qRT-PCR and Western blotting. Monocytes that adhered to LPS-stimulated endothelial cells were measured by monocyte adhesion assay. Tight junctional factors were detected by qRT-PCR (Claudin 5 and Occludin) and immunofluorescence staining (Claudin 5 and ZO-1). The permeability of the hCMEC monolayer was determined by fluorescence spectrophotometry of transmembrane fluorescin and transendothelial electrical resistance (TEER). NF-kB activation was measured by luciferase assay.EGCG significantly suppressed the LPS-induced expression of IL-1¦Â and TNF-¦Á in hCMECs. EGCG also inhibited the expression of MCP-1/CCL2, VCAM-1 and ICAM-1. Functional analysis showed that EGCG induced the expression of tight junction proteins (Occludin and Claudin-5) in hCMECs. Investigation of the mechanism showed that EGCG had the ability to inhibit LPS-mediated NF-¦ÊB activation. In addition, 67-kD laminin receptor was involved in the anti-inflammatory effect of EGCG.Our results demonstrated that LPS induced inflammatory cytokine production in hCMECs, which could be attenuated by EGCG. These data indicate that EGCG has a therapeutic potential for endotoxin-mediated endothelial inflammation.
%K 67LR
%K endothelial
%K (£¿)-epigallocatechin gallate
%K LPS
%K NF-¦ÊB
%U http://www.jneuroinflammation.com/content/9/1/161/abstract