%0 Journal Article
%T Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods
%A H. Fukushima
%A S. Shimizu
%A Y. Inatsu
%J Journal of Pathogens
%D 2011
%I Hindawi Publishing Corporation
%R 10.4061/2011/735308
%X Yersinia enterocolitica and Y. pseudotuberculosis which can cause yersiniosis in humans and animals are thought to be significant food-borne pathogens and be important as hygiene indicator in food safety. The pathogenic Y. enterocolitica serotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 27/2, O:8/1b, and O:9/2, have been reported worldwide. Y. pseudotuberculosis is distributed less widely than Y. enterocolitica. Isolation methods usually involve selective and recovery enrichment of the food sample followed by plating onto selective media, confirmation of typical colonies and testing for virulence properties of isolated strains. Recently, DNA-based methods, such as PCR assays, have been developed to detect pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods more rapidly, and sensitivity than can be achieved by conventional culture methods. This paper reviews commercially available conventional and PCR-based procedures for the detection of pathogenic Yersinia in food. These methods are effective as the isolation and detection methods to target pathogenic Y. enterocolitica and Y. pseudotuberculosis in foods. 1. Overview Food-borne pathogenic Yersinia (Y. enterocolitica and Y. pseudotuberculosis) is facultative anaerobic, gram-negative Enterobacteriaceae and is isolated frequently from soil, water, animals, and foods [1¨C4]. Y. enterocolitica causes human infections whose symptoms include diarrhea, terminal ileitis, mesenteric lymphadenitis, arthritis, and septicemia. Y. pseudotuberculosis causes mesenteric lymphadenitis, diarrhea, and septicemia in humans. As a psychrophilic organism, Yersinia is able to grow at 4¡ãC, and cold chain food products could offer a potential food safety hazard [3, 5, 6]. The pathogenic Y. enterocolitica serotypes/biotypes are O:3/4 and 3 variant VP negative, O:5, 27/2, O:8/1b, and O:9/2 have been reported worldwide [7, 8]. In Japan, O:3/3 variant VP negative is the most frequent cause of human yersiniosis [8]. In the United States, despite declining incidences of serotype O:8/1b infections, O:3/4 and O:5, 27/2 infections are on the increase [7]. In Europe, Serotype O:3 and O:9 infections account for over 90% of Y. enterocolitica infections. Y. pseudotuberculosis is distributed less widely than Y. enterocolitica and, although frequently isolated from animals, is rarely isolated from soil, water, and food [9¨C12]. A large outbreak of Y. pseudotuberculosis infection has been reported in Japan [13, 14]. In the Far East including Japan, Y. pseudotuberculosis various serotypes (1b, 2a, 2b, 2c, 3, 4a, 4b, 5a, 5b,
%U http://www.hindawi.com/journals/jpath/2011/735308/