%0 Journal Article %T DEVELOPMENT OF HIGH VALUE PHYTOCHEMICALS FROM GREEN TOBACCO %A Mahendra %A K %A Narasimha Rao %A C.V %A Murthy %A Y.L.N %A Bala Murali Krishna %A K %J International Journal of Chemical Sciences and Research %D 2011 %I %X In Indian agriculture, tobacco has a prominent place. Tobacco could be developed as an important food crop in combination with its traditional use for smoking and chewing. Use of tobacco for either smoking or chewing purpose is viewed seriously due to alleged health factors. The chemistry of tobacco is unique with the presence of a wide spectrum of chemical compounds of which nicotine, solanesol, malic and citric acid were identified as potential chemicals which could be recovered and converted to value-added products. This situation necessitated to examine the green tobacco crop as a source for recovery of phytochemicals alone. With the objective of maximizing bio-mass production for optimum recovery of proteins, nicotine, solanesol and organic acids from green tobacco, a field experiment was conducted by manipulating agronomic practices. Flue-cured tobacco variety was planted to have a high density population. Green leaf was harvested in six pauses for processing. An integrated approach was developed for extraction of above phytochemicals from green leaf and recovery potential was estimated. The green lamina was macerated and filtered through a cheese cloth, the green liquid was heat coagulated at 80ˇăC to precipitate the crude protein along with chloroplast protein. Remaining after protein separation, brown liquid containing nicotine, was passed through a strongly cationic-exchange resin. The wet green mass left over after filtration is subjected to hexane extraction for the recovery of solanesol. Processing of chloroplast fraction for recovery of solanesol will be standardized. The column effluent after nicotine recovery is treated with lime to precipitate organic acids. Results indicated that tobacco cultivation for phytochemicals is a prospective proposition. The need to evolve proper marketing systems for the phytochemicals or their value-added products is also discussed %K Green tobacco %K Phytochemicals %K Solanesol %K Coenzyme Q10. %U http://www.ijcsr.co.in/Documents/Volumes/Vol%20I%20issue%201/ijcsr2011010102.pdf