%0 Journal Article
%T Effect of essential fatty acids on glucose-induced cytotoxicity to retinal vascular endothelial cells
%A Junhui Shen
%A Shengrong Shen
%A Undurti N Das
%A Guotong Xu
%J Lipids in Health and Disease
%D 2012
%I BioMed Central
%R 10.1186/1476-511x-11-90
%X RF/6A cells were treated with different concentrations of high glucose, ¦Á-linolenic acid and linoleic acid and Zn2+. The alterations in mitochondrial succinate dehydrogenase enzyme activity, cell membrane fluidity, reactive oxygen species generation, SOD enzyme and vascular endothelial growth factor (VEGF) secretion were evaluated.Studies showed that hyperglycemia-induced excess proliferation of retinal vascular endothelial cells can be abrogated by both linoleic acid (LA) and ¦Á-linolenic acid (ALA), while the saturated fatty acid, palmitic acid was ineffective. A dose¨Cresponse study with ALA showed that the activity of the mitochondrial succinate dehydrogenase enzyme was suppressed at all concentrations of glucose tested to a significant degree. High glucose enhanced fluorescence polarization and microviscocity reverted to normal by treatment with Zn2+ and ALA. ALA was more potent that Zn2+. Increased level of high glucose caused slightly increased ROS generation that correlated with corresponding decrease in SOD activity. ALA suppressed ROS generation to a significant degree in a dose dependent fashion and raised SOD activity significantly. ALA suppressed high-glucose-induced VEGF secretion by RF/6A cells.These results suggest that EFAs such as ALA and LA may have beneficial action in the prevention of high glucose-induced cellular damage.
%K ¦Á-linolenic acid
%K Diabetic retinopathy
%K Oxidative stress
%K Membrane fluidity
%U http://www.lipidworld.com/content/11/1/90/abstract