%0 Journal Article
%T Efecto neuroprotector del extracto hidroalcoh車lico de Piper aduncum ※matico§ en un modelo in vitro de neurodegeneraci車n The neuroprotective effect of a hydroalcoholic extract of Piper aduncum ※matico§ in an in vitro model of neurodegeneration
%A C谷sar Zaa
%A Martha Valdivia
%A 芍lvaro Marcelo
%J Revista Peruana de Biolog赤a
%D 2013
%I Ciudad Universitaria, Lima
%X Durante los procesos neurodegenerativos la funci車n y viabilidad de las neuronas se reduce. En particular, el incremento patol車gico en la concentraci車n de calcio intracelular, la alteraci車n de la plasticidad sin芍ptica y apoptosis est芍n implicados en la Enfermedad de Alzheimer (EA), paradigma de un proceso neurodegenerativo, relacionado con la p谷rdida progresiva de las funciones cognitivas. Para evaluar el efecto neuroprotector de Piper aduncum※matico§ se indujo el da o con el p谷ptido A汕 a c谷lulas cultivadas. Igualmente, c谷lulas hipocam-pales fueron tratadas con el A汕, y se evalu車 viabilidad celular, niveles de caspasa-3 y expresi車n de receptores NMDA en sinapsis. Tambi谷n se registr車 el influjo de calcio intracelular en tratamientos con agonista NMDA y P. aduncum. En la evaluaci車n neuroprotectora hay una reducci車n de un 20,6% de caspasa-3; un aumento del 9,6% por encima del control y una recuperaci車n del 20,86% para las prote赤nas NR1 y SV2 respectivamente. Adem芍s hay una reducci車n de m芍s del 50% del calcio celular. Estos resultados evidencian efecto neuropro-tector de P. aduncumpara el modelo estudiado. During the neurodegenerative processes there is a reduction of the function and viability of neurons. The pathological increase in the intracellular calcium concentration, the alteration of the synaptic plasticity and the apoptosis are implicated in Alzheimer disease (AD, a paradigm of a neurodegenerative process, related with the progressive loss of cognitive functions. To evaluate neuroprotector effect of Piper aduncumdamage was induced with A汕1-42 and NMDA to cultured cells. So hippocampal cells were treated with A汕 1-42 1 uM and cellular viability, levels of caspasa-3 and expression of NMDA receptors in synapses were evaluated. Also, intracellular calcium influxe (sobreestimulation with NMDA) of was registered in treatments with P. aduncum. In the neuroprotector evaluation there is a reduction of 20.6% of caspasa-3; one increase of 9.6% over control and recovery of 20.86% for NR1 and SV2 proteins respectively. Besides, there is a reduction of more than 50% of cellular calcium. These results demonstrate neuroprotector effect of P. aduncumfor the studied model.
%K Piper aduncum
%K neuroprotecci車n
%K neurodegeneraci車n
%K apoptosis
%K sinapsis.
%U http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/997