%0 Journal Article
%T Hydrogen-rich water inhibits glucose and ¦Á,¦Â -dicarbonyl compound-induced reactive oxygen species production in the SHR.Cg-Leprcp/NDmcr rat kidney
%A Masanori Katakura
%A Michio Hashimoto
%A Yoko Tanabe
%A Osamu Shido
%J Medical Gas Research
%D 2012
%I BioMed Central
%R 10.1186/2045-9912-2-18
%X Kidney homogenates from Wistar rats were incubated in vitro with glucose and ¦Á,¦Â-dicarbonyl compounds containing HRW, following which ROS levels were measured. In vivo animal models of metabolic syndrome, SHR.Cg-Leprcp/NDmcr rats, were treated with HRW for 16£¿weeks, following which renal ROS production and plasma and renal ¦Á,¦Â-dicarbonyl compound levels were measured by liquid chromatograph mass spectrometer.HRW inhibited glucose- and ¦Á,¦Â-dicarbonyl compound-induced ROS production in kidney homogenates from Wistar rats in vitro. Furthermore, SHR.Cg-Leprcp/NDmcr rats treated with HRW showed a 34% decrease in ROS production. Moreover, their renal glyoxal, methylglyoxal, and 3-deoxyglucosone levels decreased by 81%, 77%, and 60%, respectively. Positive correlations were found between renal ROS levels and renal glyoxal (r£¿=£¿0.659, p£¿=£¿0.008) and methylglyoxal (r£¿=£¿0.782, p£¿=£¿0.001) levels.These results indicate that HRW inhibits the production of ¦Á,¦Â-dicarbonyl compounds and ROS in the kidneys of SHR.Cg-Leprcp/NDmcr rats. Therefore, it has therapeutic potential for renal dysfunction in patient with type 2 diabetes and metabolic syndrome.
%K Hydrogen-rich water
%K ¦Á
%K ¦Â-dicarbonyl compounds
%K Oxidative stress
%K Metabolic syndrome model
%K Advanced glycation end products
%U http://www.medicalgasresearch.com/content/2/1/18/abstract