%0 Journal Article
%T Study on Cryo-preservation of Theileria annulata Schizont Infected Cell Line Vaccine Strain S15
%A Habibi
%A G.
%A Bozorgi
%A S.
%A Hatami
%A A.
%A Esmaeil Nia
%A K.
%J Archives of Razi Institute
%D 2012
%I Razi Vaccine and Serum Research Institute, Karaj
%X The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the conventional cryo-preservative medium containing 5% PEG treated Bovine Serum and 10% glycerol successfully cryo-preserved cell as efficiently as the standard medium containing 80% FBS and 10% glycerol and was superior to all thirteen different experimental cryo-preservative media. A crucial result was obtained among the thirteen different serum-free media which showed that the 0.2M sorbitol+10% glycerol method yielded cells with viability of 54% and good plating efficiency results. It was concluded that the conventional method would be the best and ideal for the cryo-preservation and storage of Theileria annulata-infected cells for vaccine purposes to date.
%K Cryo-preservation
%K Theileria annulata
%K Sorbitol
%K Sucrose
%K Gelatin
%K Glycerol
%U http://www.archrazi.com/browse.php?a_id=325&sid=1&slc_lang=en