%0 Journal Article
%T Molecular-genetic risk assessement of determining angiotensin-converting enzyme hyperactivity in hemorrhagic fever with renal syndrome
%A Asiya A. Baygildina
%A Denis V. Islamgulov
%A Felix Kh. Kamilov
%A Tamara A. Khabelova
%J Russian Open Medical Journal
%D 2012
%I Limited liability company ?Science and Innovations? (Saratov)
%X The present study was designed to investigate changes in angiotensin-converting enzyme (ACE) blood activity and angiotensin II type 1 receptor gene polymorphism as a possible disease predictor in hemorrhagic fever with renal syndrome (HFRS). Four hundred and nine patients (346 males and 63 females) with HFRS serologic confirmation were enrolled in the study. Their age ranged from 15 to 65 years. ACE blood activity was assessed kinetically using the B¨¹hlmann (Switzerland) kit. Peripheral blood genomic DNA was isolated by a phenol-chloroform extraction. The genotyping of DNA loci was done using a polymerase chain reaction of DNA synthesis. Statistically, ACE blood activity was significantly higher throughout the entire HFRS course with diverse severity apart from the feverish phase of moderate-to-severe uncomplicated disease forms. *A1166 and *C1166 alleles, *A1166/*A1166 and *C1166/*C1166 genotypes of angiotensin II type 1 receptor gene were not associated with HFRS severity. The results of this study indicate that high ACE activity has not adaptive characteristics due to abnormalities in angiotensin II reception. It is an adequate metabolic response of the body to endotheliotropic virus activity.
%K angiotensin-converting enzyme
%K gene of angiotensin II type 1 receptor
%K hemorrhagic fever with renal syndrome
%U http://www.romj.org/node/26