%0 Journal Article
%T Protective effect of the poly(ADP-ribose) polymerase inhibitor PJ34 on mitochondrial depolarization-mediated cell death in hepatocellular carcinoma cells involves attenuation of c-Jun N-terminal kinase-2 and protein kinase B/Akt activation
%A Balazs Radnai
%A Csenge Antus
%A Boglarka Racz
%A Peter Engelmann
%A Janos K Priber
%A Zsuzsanna Tucsek
%A Balazs Veres
%A Zsuzsanna Turi
%A Tamas Lorand
%A Balazs Sumegi
%A Ferenc Gallyas
%J Molecular Cancer
%D 2012
%I BioMed Central
%R 10.1186/1476-4598-11-34
%X We found that sublethal concentrations of IK11 prevented proliferation, migration and entry of the cells into their G2 phase. At higher concentrations, IK11 induced reactive oxygen species (ROS) production, mitochondrial membrane depolarization, activation of c-Jun N-terminal kinase 2 (JNK2), and substantial loss of HepG2 cells. ROS production appeared marginal in mediating the cytotoxicity of IK11 since N-acetyl cysteine was unable to prevent it. However, the PARP inhibitor PJ34, although not a ROS scavenger, strongly inhibited both IK11-induced ROS production and cell death. JNK2 activation seemed to be a major mediator of the effect of IK11 since inhibition of JNK resulted in a substantial cytoprotection while inhibitors of the other kinases failed to do so. Inhibition of Akt slightly diminished the effect of IK11, while the JNK and Akt inhibitor and ROS scavenger trans-resveratrol completely protected against it.These results indicate significant involvement of PARP, a marginal role of ROS and a pro-apoptotic role of Akt in this system, and raise attention to a novel mechanism that should be considered when cancer therapy is augmented with PARP-inhibition, namely the cytoprotection by inhibition of JNK2.
%K Poly(ADP-ribose)-polymerase (PARP)
%K Mitogen activated protein kinase (MAPK)
%K c-Jun N-terminal kinase (JNK)
%K Protein kinase B (PKB/Akt)
%K Mitochondrial depolarization
%U http://www.molecular-cancer.com/content/11/1/34/abstract