%0 Journal Article
%T Bone marrow-derived fibroblast growth factor-2 induces glial cell proliferation in the regenerating peripheral nervous system
%A Victor Tulio Ribeiro de Resende
%A Alvaro Carrier-Ruiz
%A Robertha M R Lemes
%A Ricardo A M Reis
%A Rosalia Mendez-Otero
%J Molecular Neurodegeneration
%D 2012
%I BioMed Central
%R 10.1186/1750-1326-7-34
%X Here we show that bone marrow-derived cell treatment induce an increase in the expression of FGF-2 in the sciatic nerve, dorsal root ganglia and the dorsolateral (DL) region of the lumbar spinal cord (LSC) in a model of sciatic nerve transection and connection into a hollow tube. SCs in culture in the presence of bone marrow derived conditioned media (CM) resulted in increased proliferation and migration. This effect was reduced when FGF-2 was neutralized by pretreating BMMC or CM with a specific antibody. The increased expression of FGF-2 was validated by RT-PCR and immunocytochemistry in co-cultures of bone marrow derived cells with sciatic nerve explants and regenerating nerve tissue respectivelly.We conclude that FGF-2 secreted by BMMC strongly increases early glial proliferation, which can potentially improve PNS regeneration.
%K Regeneration
%K Sciatic nerve
%K Dorsal root ganglia
%K Spinal cord
%K FGF-2
%K Schwann cell
%K Bone marrow derived cells
%K Proliferation
%U http://www.molecularneurodegeneration.com/content/7/1/34/abstract