%0 Journal Article
%T RP-HPLC Determination of Torsemide in Pharmaceutical Formulation by Liquid Chromatography
%A Rajesh Shukla
%J Asian Journal of Biomedical and Pharmaceutical Sciences
%D 2013
%I 
%X A high-performance liquid chromatographic method for the determination ofTorsemide is described. The assay uses a reversed-phase gradient systemand UV-detection. The chromatographic separation was carried out on a¦ÌBondapak C18 column with a mobile phase consisting ofAcetonitrile/Phosphate Buffer 0.05M (pH 2.4) in ratio of 70/30. The methodwas validated and found to be linear in the range of 50-100 ¦Ìg/ml. Theretention time of Torsemide was 6.00 ¡À 0.20 min. The chromatogramsshowed good resolution and no interference with impurity. The meanrecovery of the Torsemide was found to be above 99.9%. Both accuracy andprecision data showed good reproducibility. The linearity range was found tobe 50-100 ¦Ìg/ml with coefficient of variation of 0.998 at calibration point.The limit of detection and limit of quantization for Torsemide were found tobe 148.1ng and 448.9ng respectively. These results suggested that theanalytical method was linear, precise, and accurate
%K HPLC
%K Acetonitrile
%K isocratic
%K Torsemide
%U http://www.jbiopharm.com/index.php/ajbps/article/view/151