%0 Journal Article
%T Mitogen activated protein kinase phosphatase-1 prevents the development of tactile sensitivity in a rodent model of neuropathic pain
%A Christian Ndong
%A Russell P Landry
%A Joyce A DeLeo
%A E. Alfonso Romero-Sandoval
%J Molecular Pain
%D 2012
%I BioMed Central
%R 10.1186/1744-8069-8-34
%X We cloned rat spinal cord MKP-1 and optimize MKP-1 cDNA in vitro using transfections to BV-2 cells. We observed that in vitro overexpression of MKP-1 blocked lipopolysaccharide-induced phosphorylation of p38 (and other MAPKs) as well as release of pro-algesic effectors (i.e., cytokines, chemokines, nitric oxide). Using this cDNA MKP-1 and a non-viral, in vivo nanoparticle transfection approach, we found that spinal cord overexpression of MKP-1 prevented development of peripheral nerve-injury-induced tactile hypersensitivity and reduced pro-inflammatory cytokines and chemokines and the phosphorylated form of p38.Our results indicate that MKP-1, the natural regulator of p-p38, mediates resolution of the spinal cord pro-inflammatory milieu induced by peripheral nerve injury, resulting in prevention of chronic mechanical hypersensitivity. We propose that MKP-1 is a potential therapeutic target for pain treatment or prevention.
%K Phosphatases
%K MKP-1
%K Spinal cord
%K p38
%K Kinases
%K Allodynia
%K Nanoparticle
%K Nanotechnology
%U http://www.molecularpain.com/content/8/1/34/abstract