%0 Journal Article
%T Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone
%A Ryan S O'Dell
%A Candida JM Ustine
%A David A Cameron
%A Sean M Lawless
%A Rebecca R Williams
%A Warren R Zipfel
%A Eric C Olson
%J Neural Development
%D 2012
%I BioMed Central
%R 10.1186/1749-8104-7-25
%X The total neurite arbor sizes of neurons in Reelin-deficient (reeler mutant) and Dab1-deficient (Reelin-non-responsive scrambler mutant) cortices were quantified and unexpectedly were not different than control arbor lengths (p£¿=£¿0.51). For each mutant, however, arbor organization was markedly different: mutant neurons manifested more primary processes (neurites emitted directly from the soma) than wild type, and these neurites were longer and displayed less branching. Reeler and scrambler mutant neurites extended tangentially rather than radially, and the Golgi apparatus that normally invests the apical neurite was compact in both reeler and scrambler mutants. Mutant cortices also exhibited a neurite ¡°exclusion zone¡± which was relatively devoid of L6 neuron neurites and extended at least 15£¿¦Ìm beneath the pial surface, an area corresponding to the marginal zone (MZ) in the wild type explants. The presence of an exclusion zone was also indicated in the orientation of mutant primary neurite and neuronal somata, which failed to adopt angles within ~20£¿ of the radial line to the pial surface. Injection of recombinant Reelin to reeler, but not scrambler, mutant cortices fully rescued soma orientation, Golgi organization, and dendritic projection defects within four hrs.These findings indicate Reelin promotes directional dendritic growth into the MZ, an otherwise exclusionary zone for L6 neurites.
%K Preplate
%K Neurite
%K Orientation
%K Epilepsy
%K Migration
%K Cortex
%K Dendrite
%U http://www.neuraldevelopment.com/content/7/1/25/abstract