%0 Journal Article
%T Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey
%A S¨¹heyla S¨¹r¨¹c¨¹o£¿lu
%A Selami G¨¹nal
%A Nuri £¿zk¨¹t¨¹k
%A Can Bi£¿men
%J Balkan Medical Journal
%D 2012
%I Aves Yayincilik
%X Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results: The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions: The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.
%K Mycobacterium tuberculosis
%K drug resistance
%K genotyping
%K epidemiology
%U http://balkanmedicaljournal.org/text.php3?id=854