%0 Journal Article
%T Stone Pine Volatiles and Host Selection by Tomicus destruens (Wollaston) (Coleoptera: Curculionidae, Scolytidae) Vol¨¢teis de Pinheiro-Manso e Sele  o do Hospedeiro pela Hil¨¦sina Tomicus destruens (Wollaston) (Coleoptera: Curculionidae, Scolytidae)
%A Massimo Faccoli
%A Gianfranco Anfora
%A Marco Tasin
%J Silva Lusitana
%D 2011
%I Esta??o Florestal Nacional
%X Mature adults of the pine shoot beetle Tomicus destruens reproduce in trunks of dying Mediterranean pines. However, there is no information about chemical stimuli governing host selection by T. destruens. The aims of this study were: 1) to identify volatiles released by the bark of stone pines (Pinus pinea) behaviorally and electro-physiologically active on T. destruens; 2) to verify which blends and concentrations of such volatiles act differently on males and females. A four-arm olfactometer was used to test behavior of walking adults of T. destruens in relation to various sources of volatiles, including bark, its collected volatiles and a synthetic blend. For each odor, the behavior of both males and females was recorded individually. Bark extracts were then analyzed by coupled gas chromatography and mass spectrometry (GC-MS) and tested by gas-chromatography coupled with electro-antennography (GC-EAD) on male and female antennae of T. destruens. In addition, a blend of synthetic compounds chosen from among those inducing EAD responseswas tested in the olfactometer at five concentrations. Behavior and EAD responses were not affected by sex, with males and females showing similar results. Five compounds extracted from bark were active on T. destruens antennae: limonene, ¦Â-caryophyllene, ¦Á-pinene, ¦Â-myrcene, and ¦Á-terpinolene. Among extracted volatiles, limonene and ¦Â-caryophyllene were known to be repellent to T. destruens, whereas attraction to a synthetic blend of ¦Á-pinene, ¦Â-myrcene and ¦Á-terpinolene was positively correlated with concentration, although a repellent effect was noted at the highest concentrations. Os adultos da hil¨¦sina Tomicus destruens reproduzem-se em troncos de v¨¢rias esp¨¦cies de pinheiros da regi o mediterranica. No entanto, n o se conhecem os est¨ªmulos qu¨ªmicos que condicionam a respetiva sele  o dos hospedeiros. Os objetivos do presente estudo s o: 1) identificar os vol¨¢teis da casca do pinheiro-manso (Pinus pinea) que influenciam a resposta eletrofisiol¨®gica e comportamental de T. destruens e 2) determinar quais as misturas e concentra  es de odores que afetam diferencialmente os dois sexos. A atratividade dos adultos da hil¨¦sina em rela  o aos vol¨¢teis (incluindo casca, vol¨¢teis da casca e uma mistura sint¨¦tica) foi estudada usando um olfat¨®metro de quatro bra os. Para cada odor o comportamento de machos e f¨ºmeas foi estudado individualmente. Amostras de casca foram analisadas atrav¨¦s de cromatografia gasosa e espectrometria de massa (GC-MS), tendo sido testados em machos e f¨ºmeas de T. destruens atrav¨¦s de cromatografia gas
%K Sele  o de hospedeiro
%K cairomonas
%K eletrofisiologia
%K olfatometria
%K terpenos
%K Pinus
%K Blastophagus
%K Host selection
%K kairomones
%K electrophysiology
%K olfactometry
%K terpenes
%K Pinus
%K Blastophagus
%U http://www.scielo.oces.mctes.pt/scielo.php?script=sci_arttext&pid=S0870-63522011000200008