%0 Journal Article
%T Higher frequency of chromosome aberrations in lymphocytes, exposed and non-exposed to mytocmycin C, amongst postmenopausal obese women compared to non-obese from the department of Cauca, Colombia Mayor frecuencia de aberraciones cromos車micas en linfocitos expuestos o no a mitomicina C, de mujeres post-menop芍usicas obesas en comparaci車n con mujeres no obesas del departamento del Cauca, Colombia
%A Yaliana Tafurt-Cardona
%A Leidy D. Jaramillo-Ruiz
%A Wilson Muˋoz-Ordoˋez
%A Sulma L. Muˋoz-Ben赤tez
%J Biom谷dica
%D 2012
%I Instituto Nacional de Salud
%X Introduction. Epidemiological studies indicate that obesity is associated in as much as 25-20% with several types of cancer. Objective. To evaluate the frequency of chromosome aberrations in lymphocytes from postmenopausal obese and non-obese women by means of the challenge assay as biomarker of genomic instability. Materials and methods. A total of 20 obese and 20 non-obese women, all post-menopause, were recruited for the study. The groups were matched according to age (㊣ 5 years) and place of origin. After freely signing a consent form, women were interviewed using a structured questionnaire and a blood sample (5 cc) was drawn in vacutainer tubes. From each sample, lymphocyte cell cultures were established with and without mitomycin C (challenge assay). Afterwards, the frequency of chromosome aberrations were registered for each group and treatment. Data were analyzed using the statistical SPSS v. 14.0. Results. In general, obese women had a higher chromosome aberrations' frequency when compared to non-obese women. After exposing the cell cultures to Mitomycin C, obese women presented an increase in the number of total chromosome aberrations in comparison to non-obese women (3,74 ㊣ 0,63 vs. 2,70 ㊣ 0,61; p = 0,001). Conclusions. The higher frequency of chromosome aberrations in lymphocytes from postmenopausal obese women compared to non-obese women suggest differences in the DNA repair capacity, which could explain the association between genomic instability and the higher incidence of cancer in this population. Introducci車n. Estudios epidemiol車gicos indican que la obesidad est芍 asociada en un 25-30% con varios tipos de c芍ncer. Objetivo. Evaluar la frecuencia de aberraciones cromos車micas en linfocitos de mujeres post-menop芍usicas obesas y no-obesas, mediante la prueba de challenge como biomarcador de inestabilidad gen車mica. Materiales y m谷todos. Un total de 40 mujeres post-menop芍usicas fueron reclutadas para el estudio (20 obesas y 20 no-obesas). Los grupos fueron pareados seg迆n edad (㊣ 5 a os) y procedencia. Despu谷s de la firma voluntaria del consentimiento informado, las mujeres fueron entrevistadas y se tom車 una muestra de sangre perif谷rica (5 cc). Se establecieron cultivos de linfocitos con y sin tratamiento a mitomicina C (prueba de challenge) y posteriormente se registr車 la frecuencia de aberraciones cromos車micas para cada grupo y tratamiento. Resultados. En general, las mujeres obesas presentaron una mayor frecuencia de aberraciones cromos車micas en comparaci車n con las mujeres no-obesas. Despu谷s de exponer los cultivos celulares a mitomicina C,
%K Obesidad
%K menopausia
%K neoplasias
%K inestabilidad cromos車mica
%K aberraciones cromos車micas
%K Colombia
%U http://www.revistabiomedica.org/index.php/biomedica/article/view/412