%0 Journal Article
%T Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)
%A Golandam Sharifi
%A Hassan Ebrahimzadeh
%A Behzad Ghareyazie
%A Javad Gharechahi
%A Elaheh Vatankhah
%J Proteome Science
%D 2012
%I BioMed Central
%R 10.1186/1477-5956-10-3
%X We have compared proteome profiles of non-embryogenic and embryogenic calli with native corm explants. Total soluble proteins were phenol-extracted and loaded on 18 cm IPG strips for the first dimension and 11.5% sodium dodecyl sulfate-polyacrylamide gels for the second dimension. Fifty spots with more than 1.5-fold change in abundance were subjected to mass spectrometry analysis for further characterization. Among them 36 proteins could be identified, which are classified into defense and stress response, protein synthesis and processing, carbohydrate and energy metabolism, secondary metabolism, and nitrogen metabolism.Our results showed that diverse cellular and molecular processes were affected during somatic to embryogenic transition. Differential proteomic analysis suggests a key role for ascorbate metabolism during early stage of SE, and points to the possible role of ascorbate-glutathione cycle in establishing somatic embryos.Saffron (Crocus sativus L., Iridaceae) has long been cultivated for the production of saffron spice, which makes it interesting from an economic as well as a scientific point of view. Saffron is an autumn flowering species and an auto-triploid (2n = 3x = 24) form of a species found in eastern Greece. An origin in Western or central Asia (possibly Iran) is suspected [1]. In vitro culture of saffron is a promising approach for making the commercial production of crocin, safranal and picrocrocin (the flavor and coloring characteristic of saffron) less expensive than conventional means i.e. through manual harvesting of styles [2]. Somatic embryogenesis (SE) has also been recognized as a promising approach for the regeneration of plantlets in tissue cultures and as a vegetative propagation system in vitro. The ability of plant cells to produce somatic embryos in culture, made SE not only as an interesting issue for genetic engineering and biotechnology but also as a model system for studying zygotic embryogenesis [3,4]. Several potential biot
%K Saffron
%K Crocus sativus L.
%K somatic embryogenesis
%K two-dimensional gel electrophoresis
%K MALDI-TOF/TOF
%U http://www.proteomesci.com/content/10/1/3