%0 Journal Article
%T Acid Hydrolysis of Pectin for Cell Growth of Cupriavidus necator
%A Gabriel Locatelli
%A Gabriella Dias da Silva
%A Leandro Finkler
%A Christine Lamenha Luna Finkler
%J Biotechnology
%D 2012
%I Asian Network for Scientific Information
%X The effect of acid concentration (1-7%, v/v) and temperature (70-100C) on the pectin hydrolysis was studied using pectin at 1% (w/v) for 4 h hydrolysis. Experiments were arranged according to a central composite statistical design and Response Surface Methodology (RSM) was used to assess factor interactions and empirical models on response variable (reducing groups concentration - RGC). The highest RGC (6.5 g L-1) was achieved at 100C and 1% (v/v) H2SO4. Kinetics of RGC release was also performed and hydrolysate was used as carbon source for cell growth of Cupriavidus necator. The maximum specific growth rate and the substrate-to-cell conversion factor were 0.26 h-1 and 0.55 g g-1, respectively an indicative of that pectin hydrolysate is a potential substrate for cell growth of this microorganism.
%K experimental design
%K reducing group concentration
%K Pectin
%K Cupriavidus necator
%K acid hydrolysis
%U http://docsdrive.com/pdfs/ansinet/biotech/2012/29-36.pdf