%0 Journal Article
%T Bioanalytical method development and validation of bupropion hydrochloride in rat plasma by RP-HPLC
%A Thakar D.S
%A Varghese A
%J International Journal of Pharma Bioscience and Technology
%D 2013
%I IJPBST
%X A novel, rapid, sensitive, accurate and specific HPLC assay with UV-Visible detection (250 nm) was developed and validated for the determination of bupropion hydrochloride in rat plasma. Phenacetin was used as internal standard (IS). The plasma proteins were precipitated by a single step protein precipitation extraction procedure using methanol (acidic pH). Chromatographic separation was achieved with a combination of acetonitrile and 0.01 M potassium dihydrogen phosphate (pH 3.0 adjusted with orthophosphoric acid) in the gradient mode on a C18 (250 mm ¡Á 4.6 mm, 5 ¦Ìm) analytical column. Mean recovery of bupropion hydrochloride from rat plasma was around 55 % for 2.5-50 ¦Ìg/ml concentrations. The assay exhibited good linear relationship with an r2 of 0.9999. Lower limit of Quantification limit (LLOQ) was 1.84 ¦Ìg/ml of bupropion hydrochloride and accuracy and precision were over the concentration range 2.5-50 ¦Ìg/ml. The method was validated with good sensitivity, accuracy, precision and recovery. The assay can be applied successfully to pharmacokinetic studies.
%K Bupropion HCl
%K HPLC
%K Bioanalysis
%K Rat plasma
%K UV detection.
%U http://www.ijpbst.com/ijpdstfiles/Ijpbst_2013_01_04.pdf