%0 Journal Article
%T Non-typeable Haemophilus influenzae decreases cilia beating via protein kinase C epsilon
%A Kristina L Bailey
%A Tricia D LeVan
%A Daniel A Yanov
%A Jacqueline A Pavlik
%A Jane M DeVasure
%A Joseph H Sisson
%A Todd A Wyatt
%J Respiratory Research
%D 2012
%I BioMed Central
%R 10.1186/1465-9921-13-49
%X NTHi supernatant exposure significantly and rapidly decreased CBF in a dose-dependent manner within 10 minutes of exposure. After 3 hours of exposure, the number of motile ciliated cells significantly decreased. Direct measurement of PKC£¿ activity revealed a dose-dependent activation of PKC£¿ in response to NTHi supernatant exposure. Both CBF and PKC£¿ activity changes were only observed in fresh NTHi culture supernatant and not observed in exposures to heat-inactivated or frozen supernatants.Our results suggest that CBF slowing observed in response to NTHi is consistent with the stimulated activation of PKC£¿. Ciliated cell detachment is associated with PKC£¿ autodownregulation.
%U http://respiratory-research.com/content/13/1/49/abstract