%0 Journal Article
%T X-ray radiation at low doses stimulates differentiation and mineralization of mouse calvarial osteoblasts
%A Soon-Sun Park1
%A #
%A Kyoung-A Kim2
%A #
%A Seung-Youp Lee1
%A Shin-Saeng Lim3
%A Young-Mi Jeon1 & Jeong-Chae Lee1
%A 3
%A *
%J BMB Reports
%D 2012
%I Korean Society for Biochemistry and Molecular Biology
%X Radiotherapy is considered to cause detrimental effects on bonetissue eventually increasing bone loss and fracture risk. However,there is a great controversy on the real effects of irradiation itselfon osteoblasts, and the mechanisms by which irradiation affectsosteoblast differentiation and mineralization are not completelyunderstood. We explored how X-ray radiation influences differentiationand bone-specific gene expression in mouse calvarialosteoblasts. Irradiation at 2 Gy not only increased differentiationand mineralization of the cells, but also upregulated the expressionof alkaline phosphatase, type I collagen, osteopontin,and osteocalcin at early stages of differentiation. However, irradiationat higher doses (£¾2 Gy) did not stimulate osteoblast differentiation,rather it suppressed DNA synthesis by the cells withouta toxic effect. Additional experiments suggested that transforminggrowth factor-beta 1 and runt-transcription factor 2 play importantroles in irradiation- stimulated bone differentiation by acting asupstream regulators of bone-specific markers.
%K Bone-formation regulatory factors
%K Ionizing irradiation
%K Mineralization
%K Mouse calvarial osteoblasts
%K TGF-¦Â1
%U http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMCU1RDEyMTAyOTIxMThfJTI4NTcxLTU3NiUyOUJNQl8xMi0xMDEucGRm