%0 Journal Article
%T A comparative study on total phenolic content, reducing power and free radical scavenging activity of aerial parts of Barleria prionitis
%A Sunil K. Jaiswal
%A Mukesh K. Dubey
%A Sanjeeb Das
%A Arti Raj Verma
%J International Journal of Phytomedicine
%D 2011
%I Advanced Research Journals
%X Context: Barleria prionitis L. (Family Acanthaceae; commonly known as Vajradanti), is an annual shrub, 1¨C3 feet high, found throughout tropical Asia and in South Africa.Objective: The aim of the present study was to evaluate the antioxidant activity of 50% ethanolic extract of leaf, flower, and stem of B. prionitis by using ¦Â carotene bleaching assay, reducing power and free radical scavenging activity (DPPH and hydroxyl radical scavenging activity). Total phenolic content (TPC) was analysed by the Folin¨CCiocalteu colorimetric method using gallic acid as standard and expressed as mg/g gallic acid equivalent (GAE).Results: Total phenolic content (TPC) and antioxidant activity (AOA) in B. prionitis leaves were found to be 67.48 mg/g GAE dry plant material and 79.20%. The B. prionitis leaves exhibited strong free radical scavenging activity as evidenced by the low IC50 values in both DPPH (1,1-diphenyl-2-picryl hydrazyl) (336.15 ¦Ìg/ml) and hydroxyl radical (568.65 ¦Ìg/ml) methods.Conclusions: The leaf of B. prionitis possesses high phenolic content, potential antioxidant activity, reducing power & radical scavenging activity in comparison to flower and stem. Keywords: Barleria prionitis, total phenolic content, ¦Â carotene bleaching assay, reducing power, free radical scavenging activity.
%U http://www.arjournals.org/index.php/ijpm/article/view/102