%0 Journal Article
%T In vitro Activity of Ampicillin Against ¦Â-Lactamase Producing Enterobacterial Species
%A A.O. Shakak
%A H.A. Saeed
%J Current Research in Bacteriology
%D 2010
%I 
%X This study was undertaken to determine the in vitro activity of ampicillin against ¦Â-lactamase producing enterobacterial species. The study was conducted during the period December 2006-August 2009. Two hundred patients were investigated. Clinical specimens collected were 38.5% ear swabs, 36.5% wound swabs and 25% urine specimens. Cultivation of these specimens on enriched and/or differential media yielded 189 bacterial isolates, of which 96 were enterobacterial species. Among the enterobacterial isolates 62.5% were ¦Â-lactamase producers as indicated by ¦Â-lactamase acidometric method. Ampicillin activity against ¦Â-lactamase producers was determined by modified Kirby-Bauer Disc Diffusion technique. The technique was judged and approved by the National Committee for Clinical Laboratory Standards (NCCLS). The results showed that positive ¦Â-Lactamase isolates were E. coli 21.6%, K. pneumoniae 15.5%, P. mirabilis 13.7% and P. vulgaris 0.9%. The result indicated that only 8.6% of the ¦Â-lactamase producers were sensitive to ampicillin while 91.4% were resistant. It has been concluded that further studies are needed to validate the usefulness of ampicillin in treatment of enterobacterial infections in Sudanese patients.
%K B-lactamase
%K enterobacterial species
%K Ampicillin
%U http://docsdrive.com/pdfs/ansinet/crb/2010/42-45.pdf