%0 Journal Article
%T How effective is T-DNA insertional mutagenesis in Arabidopsis?
%A Yi Hong Wang
%J Journal of Biochemical Technology
%D 2008
%I Sevas Publication
%X To evaluate the effectiveness of T-DNA insertion in knocking out agene, 1084 published Arabidopsis thaliana insertion mutantsrepresenting 755 genes in 648 publications were reviewed. Insertionin the protein-coding region of a gene generates a knockout at least90% of the time or 25% of the time if it is before the start codonwhich also produces a knockdown 67% of the time. Insertion afterthe stop codon had no effect on transcription of the upstream gene17% of the time compared to 8% in insertion before the start codon.T-DNA insertion can also cause deletion and translocation.
%K Arabidopsis thaliana
%K deletion
%K expression
%K knockout
%K T-DNA insertion
%K translocation
%U http://www.jbiochemtech.com/index.php/jbt/article/view/22