%0 Journal Article
%T Determinant factors for hybridmyeloma culture (H9r9) in the yield of anti - D ¨C impact of medium (IMDM and RPMI), serum (FCS) and cell density
%A Sathya R
%A Mathialagan A
%A Flora Bai B
%A Ramalingam K*
%J Journal of Biochemical Technology
%D 2008
%I Sevas Publication
%X The hybridoma (H9r9) were cultured in roller bottles with two mediaviz IMDM and RPMI with FCS (3% & 5%). The parameters such ascell count, cell viability and avidity were determined on a timecourse basis from day 1 to day 18, to assess the optimization of theroller bottle process for the production of anti ¨C D and to determinethe influence of factors namely medium, serum concentration andvarying cell density. The cell viability and cell count remainedsignificant upto 8 or 10 days. Cell viability assessed by dye bindingrevealed that apoptosis of hybridoma cells sets suddenly after 10days in all culture bottles. The observations implied that 0.2 ¡Á 106 /ml seemed to be optimum for growth of hybridoma. IMDM seemedto be comparatively superior to RPMI. The study also warrantsempirical informations of H9r9 cell metabolism to achieveoptimization.
%K Hybridoma (H9r9)
%K Foetal calf serum (FCS)
%K Anti ¨C D
%K Apoptosis
%K IMDM/RPMI Medium.
%U http://www.jbiochemtech.com/index.php/jbt/article/view/33