%0 Journal Article
%T Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis thaliana
%A So Youn Won
%A Shengben Li
%A Binglian Zheng
%A Yuanyuan Zhao
%A Dongming Li
%A Xin Zhao
%A Huilan Yi
%A Lei Gao
%A Thanh T Dinh
%A Xuemei Chen
%J Silence
%D 2012
%I BioMed Central
%R 10.1186/1758-907x-3-6
%X We introduced a dual 35 S promoter (d35S) driven luciferase reporter, LUCH, into Arabidopsis and isolated a line with a moderate level of luciferase activity. LUCH produced transgene-specific 24 nucleotide siRNAs and its d35S contained methylated cytosine in CG, CHG and CHH contexts. Treatment of the transgenic line with an inhibitor of cytosine methylation de-repressed luciferase activity. Mutations in several components of the RdDM pathway but not the maintenance methylation genes resulted in reduced d35S methylation, especially CHH methylation, and de-repression of luciferase activity. A mutation in MOM1, which is known to cooperate with RdDM to silence transposons, reduced d35S DNA methylation and de-repressed LUCH expression. A mutation in ROS1, a cytosine demethylation enzyme, increased d35S methylation and reduced LUCH expression.We developed a luciferase-based reporter, LUCH, which reports both DNA methylation directed by small RNAs and active demethylation by ROS1 in Arabidopsis. The moderate basal level of LUCH expression allows for bi-directional genetic screens that dissect the mechanisms of DNA methylation as well as demethylation.
%K Cytosine methylation
%K Demethylation
%K RdDM
%K MOM1
%K Transcriptional gene silencing
%K siRNA
%U http://www.silencejournal.com/content/3/1/6/abstract