%0 Journal Article %T Bio-samtools: Ruby bindings for SAMtools, a library for accessing BAM files containing high-throughput sequence alignments %A Ricardo H Ramirez-Gonzalez %A Raoul Bonnal %A Mario Caccamo %A Daniel MacLean %J Source Code for Biology and Medicine %D 2012 %I BioMed Central %R 10.1186/1751-0473-7-6 %X The utility of SAMtools is encapsulated in 3 main classes, Bio::DB::Sam, representing the alignment files and providing access to the data in them, Bio::DB::Alignment, representing the individual read alignments inside the files and Bio::DB::Pileup, representing the summarised nucleotides of reads over a single point in the nucleotide sequence to which the reads are aligned.Bio-samtools is a flexible and easy to use interface that programmers of many levels of experience can use to access information in the popular and common SAM/BAM format. %K Next-generation sequencing %K DNA %K High %K Throughput %K Ruby %K Bio %K SAM %K BAM %U http://www.scfbm.org/content/7/1/6/abstract