%0 Journal Article
%T Collagen/¦Â1 integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells
%A Zeng Di
%A Ou Dong-Bo
%A Wei Ting
%A Ding Lu
%J BMC Cell Biology
%D 2013
%I BioMed Central
%R 10.1186/1471-2121-14-5
%X Background The interactions between stem cells and extracellular matrix (ECM) mediated by integrins play important roles in the processes that determine stem cell fate. However, the role of ECM/integrin interaction in the formation of embryoid bodies (EBs) during cardiogenesis from murine induced pluripotent stem cells (miPSCs) remains unclear. Results In the present study, collagen type I and ¦Â1 integrin were expressed and upregulated synergistically during the formation of miPSC-derived EBs, with a peak expression at day 3 of differentiation. The blockage of collagen/¦Â1 integrin interaction by ¦Â1 integrin blocking antibody resulted in the production of defective EBs that were characterized by decreased size and the absence of a shell-like layer composed of primitive endoderm cells. The quantification of spontaneous beating activity, cardiac-specific gene expression and cardiac troponin T (cTnT) immunostaining showed that the cardiac differentiation of these defective miPSC-derived EBs was lower than that of control EBs. Conclusions These findings indicate that collagen/¦Â1 integrin interaction is required for the growth and cardiac differentiation of miPSC-derived EBs and will be helpful in future engineering of the matrix microenvironment within EBs to efficiently direct the cardiac fate of pluripotent stem cells to promote cardiovascular regeneration.
%K Collagen/¦Â1 integrin interaction
%K Embryoid body
%K Cardiac differentiation
%K Induced pluripotent stem cell
%U http://www.biomedcentral.com/1471-2121/14/5