%0 Journal Article
%T Correlation between PPAR¦Ã protein expression level in granulosa cells and pregnancy rate in IVF program
%A Mehdi Sahmani
%A Reza Najafipour
%A Laya Farzadi
%A Ebrahim Sakhinia
%J Iranian Journal of Reproductive Medicine
%D 2012
%I Shahid Sadoghi University of Medical Sciences of Yazd
%X Background: Peroxisome proliferative-activated receptors (PPARs) are nuclear receptors that involved in cellular lipid metabolism and differentiation. The subtype ¦Ã of the PPAR family (PPAR¦Ã) plays important roles in physiologic functions of ovaries.Objective: To determine correlation between PPAR¦Ã protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization (IVF) treatment.Materials and Methods: In this cross-sectional study, twenty-five samples of granulosa cells were collected from women referred to an IVF treatment center. PPAR¦Ã protein expression level in granulosa cells was determined in comparison with ¦Â-actin level as control gene with Western blot test. Laboratory pregnancy was determined by a rise in blood ¦Â-hCG level fourteen days after embryo transfer. Correlation analyses were used to test for associations between the oocytes and pregnancy occurrence as outcome variables and PPAR¦Ã protein expression level.Results: Correlation analysis indicated that there was no significant relationship between granulosa cells PPAR¦Ã protein level with IVF parameters including number of matured oocytes and the ratio of fertilized to matured oocytes. Comparison of granulosa cells PPAR¦Ã protein level with positive and negative laboratory pregnancy revealed also no significant relationship. Conclusion: According to the results of this study, PPAR¦Ã protein level in granulosa cells could not be directly correlated to the success rate of IVF.
%K : In-vitro fertilization
%K Peroxisome proliferative-activated receptor ¦Ã
%K Granulosa cells
%K Pregnancy rate
%U http://www.ijrm.ir/library/upload/article/af_823624313)%2089-113-3%20(sh)%20okf.pdf