%0 Journal Article
%T Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein
%A Hui-Chen Guo
%A Xiao-Ming Feng
%A Shi-Qi Sun
%A Yan-Quan Wei
%A De-Hui Sun
%A Xiang-Tao Liu
%A Zai-Xin Liu
%A Jian-Xiong Luo
%A Hong Yin
%J Virology Journal
%D 2012
%I BioMed Central
%R 10.1186/1743-422x-9-108
%X To study the potential of HMSNs for use as a protein delivery system or vaccine carriers. HMSNs were synthesized by a sol¨Cgel/emulsion(oil-in-water/ethanol) method, purified PCV2 GST-ORF2-E protein was loaded into HMSNs, and the resulting HMSN/protein mixture was injected into mice. The uptake and release profiles of protein by HMSNs in vitro were investigated. PCV2 GST-ORF2-E specific antibodies and secretion of IFN-¦Ă were detected by enzyme-linked immunosorbent assays, spleen lymphocyte proliferation was measured by the MTS method, and the percentage of CD4+ and CD8+ were determined by flow cytometry.HMSNs were found to yield better binding capacities and delivery profiles of proteins; the specific immune response induced by PCV2 GST-ORF2-E was maintained for a relatively long period of time after immunization with the HMSN/protein complex.The findings suggest that HMSNs are good protein carriers and have high potential for use in future applications in therapeutic drug delivery.
%K Hollow mesoporous silica nanoparticles (HMSNs)
%K Porcine circovirus type 2 (PCV2): ORF2
%K Delivery
%K Immunization
%K Mice
%U http://www.virologyj.com/content/9/1/108/abstract