%0 Journal Article
%T Supragingival plaque microbial analysis in reflection to caries experience
%A Mannaa Alaa
%A Carl¨¦n Anette
%A Campus Guglielmo
%A Lingstr£¿m Peter
%J BMC Oral Health
%D 2013
%I BioMed Central
%R 10.1186/1472-6831-13-5
%X Background Dental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a ¡°checkerboard DNA-DNA hybridization¡± based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation. Methods A total of 86 mothers and their children aged 4¨C6 years and 12¨C16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique. Results No significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups. Conclusions Unlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the ¡°checkerboard DNA-DNA hybridization technique¡± did not reveal any significant relations between the bacterial counts and the caries experience.
%U http://www.biomedcentral.com/1472-6831/13/5