%0 Journal Article
%T The Effects of Female Sexual Hormones and Estrogen Receptor Agonists on The NF-百B p50 and p65 Levels Following TBI in Rat Intestine
%A Zakieh KESHAVARZI
%A Mohammad KHAKSARI
%A Asadikaram GHOLAMREZA
%J Journal of Neurological Sciences
%D 2012
%I Ege University Press
%X Objectives: The purpose of this study was to evaluate the role of female sexual hormones on the intestinal NF-百B levels following traumatic brain injury (TBI) induction.Materials and methods: Diffuse TBI was induced by Marmarou method in ovariectomized (OVX) female rats. Rats randomly assigned into 10 groups: intact, sham+OVX, TBI, TBI+OVX, vehicle, estradiol (E2), progesterone (P), E2+P, PPT and DPN (estrogen receptor alpha and beta agonists, respectively). The intestinal NF-百B p50 and p65 levels were measured by ELISA method 5 days after TBI.Results: The intestinal levels of NF-百B p65 were decreased in TBI group vs intact group (P<0.001). All treatment groups had caused a significant reduction in intestinal levels of NF-百B p65 (P<0.001) vs OVX + TBI. The trauma induction doesn't have any effect on the intestinal NF-百B p50 levels. The OVX+TBI animals treated with estrogen, progesterone and estrogen+ progesterone showed a significant reduction (P<0.05) vs OVX + TBI (P<0.001). PPT had caused a significant reduction in intestinal levels of NF-百B p50 (P <0.05) compared to OVX + TBI. PPT and DPN significantly increased the intestinal levels of NF-百B p50 vs vehicle group (P <0.05).Conclusion: The binding activity of different subunits of NF-百B (p50 and p65) varies following traumatic brain injury. Also, because the NF-百B binding activity reduced in intestinal tissue after treatment with steroids, it is possible that steroids do their performance by reducing the activity of NF-百B and thereby reduce the production of intestinal inflammatory cytokines.
%K NF-百B
%K Brain injury
%K DPN
%K PPT
%K Progesterone
%U http://www.jns.dergisi.org/pdf/pdf_JNS_582.pdf