%0 Journal Article
%T Effect of extremely low frequency magnetic field in prevention of spinal cord injury-induced osteoporosis
%A Jayanand Manjhi
%A PhD
%A Suneel Kumar
%A MSc
%A Jitendra Behari
%A PhD
%A Rashmi Mathur
%A PhD
%J Journal of Rehabilitation Research and Development
%D 2013
%I Rehabilitation Research and Development Service, Department of Veterans Affairs
%R 10.1682/jrrd.2011.12.0248
%X The present study was designed to investigate the effect of extremely low frequency (ELF) magnetic field (MF) on spinal cord injury (SCI)-induced osteoporosis in rats. Adult male Wistar rats (n = 24) were equally divided into sham, SCI, and SCI+MF groups. Complete transection of spinal cord (thoracic 11 vertebra) was surgically performed under anesthesia, whereas in the sham group only laminectomy was done. Post-SCI day 1, rats were either exposed (2 h/d กม 8 wk) to ELF-MF (17.96 micro-Tesla, 50 Hz; SCI+MF group) or sham exposed (SCI group). Basso, Beattie, and Bresnahan (BBB) score was recorded weekly. All the rats were sacrificed 8 wk post-SCI; tibia and femur bones were isolated for the analysis of bone mineral content (BMC; total calcium [Ca], phosphorus [P], carbon [C]), bone mineral density (BMD), and biochemical status (osteocalcin, collagen I, alkaline phosphatase). The BBB score decreased post-SCI, which partially recovered after ELF-MF. In SCI rats, there was a statistically significant decrease in BMC, Ca, P, C, BMD, and biochemical level in both the bones as compared with the sham group, which was attenuated in SCI+MF rats except the C content. Electron microscopic study revealed the enhancement of microstructural composition and compactness in cortical and trabecular parts of treated bones. The results suggest that the chronic (2 h/d กม 8 wk) ELF-MF exposure (17.96 micro-Tesla, 50 Hz) to SCI rats is effective in attenuating SCI-induced osteoporosis.
%K alkaline phosphatase
%K BBB score
%K bone mineral contents
%K bone mineral density
%K collagen I
%K complete transection
%K extremely low frequency magnetic field
%K osteocalcin
%K osteoporosis
%K spinal cord injury
%U http://www.rehab.research.va.gov/jour/2013/501/pdf/manjhi501.pdf