%0 Journal Article
%T Reverse Phase Ultra Fast Liquid Chromatographic Method for Determination of Gemifloxacin Mesylate in Tablet Dosage Form
%A Panda SS*
%A Ravi Kumar BVV
%A Mohanta G
%A Patel PK
%J Journal of PharmaSciTech
%D 2012
%I Trinity Trust
%X A novel, accurate and precise reverse phase ultra fast liquid chromatographic method for determination of gemifloxacin mesylate has been developed and validated. Separation was achieved on an Enable C18G column (250mm ¡Á 4.6mm i.d.,5¦Ìm) using methanol: 10mM TBAHS (70:30, v/v) as mobile phase at a flow rate of 1.0ml/min and PDA detection at 271nm. Linearity was observed in the concentration range of 1.0-200 ¦Ìg/ml(r2=1). The method was validated for accuracy, precision, stability, specificity, robustness and system suitability. Forced degradation was performed by using HCl, NaOH, H2O2 , thermal and UV radiation. The method was used successfully for the determination of gemifloxacin 2 2 mesylate in tablet dosage form.
%K Gemifloxacin mesylate
%K RP-UFLC
%K TBAHS
%K stability
%U http://www.pharmascitech.in/admin/php/uploads/26_pdf.pdf