%0 Journal Article
%T Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography
%A Pedro Mesquita
%A Pedro de Sousa Gomes
%A Paula Sampaio
%A Gintaras Juodzbalys
%J Journal of Oral & Maxillofacial Research
%D 2012
%I STILUS OPTIMUS
%X Objectives: The aim of this study is to compare two commercially available screw-type sandblasted and acid-etched (SLA) Ti implant systems from Eckermann Laboratorium S.L., with similar geometry and distinct microtopography, regarding surface properties and osteoblastic cytocompatibility. Material and Methods: Implant I (referred as a conventional SLA system) and Implant II (a system patented as Eckcyte ) were characterized for macro and microtopograpphy, surface roughness and chemical composition. For the cytocompatibility studies, human bone marrow osteoblastic cells were seeded over the implants¡¯ surface, and the cell response was assessed for cell adhesion and proliferation, alkaline phosphatase (ALP) activity and matrix mineralization. Results: Implant I presented a rough surface with irregularly shaped and sized cavities among flatter-appearing areas, whereas Implant II exhibited a homogeneous rough microporous surface. Compared to Implant I, Implant II presented higher Ra values (0.8 [SD 0.008] ¦Ìm and 1.21 [SD 0.15] ¦Ìm, respectively, P < 0.05) and also increased values of Rz, Rt and Rsm, a more negative value of Rsk, and similar RKu values. XPS showed the expected presence of Ti, O, C and N; Al, Si, F, P and Ca were detected in low concentrations. Implant II exhibited significantly lower Al levels. Both implants supported the adhesion, proliferation and differentiation of osteoblastic cells. Implant II showed a thicker fibrilar cell layer and an earlier onset and more abundant matrix mineralization. Conclusions: The homogeneous rough and microporous surface of Implant II is most probably a main contributor for its improved cell response.
%K dental implants
%K surface properties
%K bone marrow
%K osteoblasts
%K differentiation cell
%K cell culture.
%U http://www.ejomr.org/JOMR/archives/2012/1/e4/v3n1e4ht.htm