%0 Journal Article
%T Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules
%A Terzi£¿ M.
%A Marinkovi£¿ B.P.
%A £¿evi£¿ D.
%A Jureta J.
%J Facta Universitatis Series : Physics, Chemistry and Technology
%D 2008
%I University of Ni?
%R 10.2298/fupct0801105t
%X Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules.
%K fluorescence
%K laser-induced spectroscopy
%K biomolecules
%K optodynamic
%U http://www.doiserbia.nb.rs/img/doi/0354-4656/2008/0354-46560801105T.pdf