%0 Journal Article
%T Fluorescence and scanning electron microscopy of citrus sinensis protoplasts
%A Paulo Celso de Mello-Farias et al.
%A Laudete Maria Sartoretto
%A Ana L¨²cia Soares Chaves
%J Evid¨ºncia : Ci¨ºncia e Biotecnologia - Interdisciplinar
%D 2008
%I Universidade do Oeste de Santa Catarina
%X The technology of protoplasts isolation is an important tool for studies of plasmatic membrane biochemistry and physiology and cell wall regeneration. Protoplasts of ¡°Caipira¡± sweet orange (Citrus sinensis L. Osbeck) were enzymatically isolated from cells in suspension and subsequently cultured in liquid medium. This study aimed to evaluate the viability of protoplasts of ¡°Caipira¡± sweet orange freshly isolated by the Fluorescein Diacetate (FDA) stain, which showed 95% of fluorescing protoplasts. Through the technique of scanning electron microscopy, it was observed protoplasts of ¡°Caipira¡± sweet orange freshly isolated. It was used Calcofluor White (CFW) fluorescent stain to visualize the cell wall regenerated protoplasts after 8 days following culture of the above-mentioned variety, wherein was observed the cell wall through a bright blue ring intense fluorescence..
%K Protoplasts
%K Cell culture
%K Fluorescence and scanning electron microscopy
%K Citrus
%U http://editora.unoesc.edu.br/index.php/evidencia/article/view/1870/942