%0 Journal Article
%T Karyotyping and in situ chromosomal localization of rDNA sites in black cumin Bunium persicum (Boiss) B. Fedtsch,1915 (Apiaceae)
%A R. K. Chahota
%A Y. Mukai
%A H.K. Chaudhary
%A Naval Kishore
%J Comparative Cytogenetics
%D 2011
%I Pensoft Publishers
%R 10.3897/compcytogen.v5i4.965
%X The fluorescent in situ hybridization (FISH) technique has been applied to somatic chromosomes in the medicinally important species, Bunium persicum, to elucidate its karyotypes. The bicolour FISH technique involving 18S-5.8S-26S and 5S ribosomal RNA genes as probes was used to assign physical localization and measurement of rDNA sites on homologous pairs of chromosomes. The two 18S-5.8S-26S rRNA gene sites were at the terminal regions of the short arms of the chromosomes 1 and 2 involving NOR region of chromosome 1. The 5S rDNA sites were found on subtelomeric region of the long arm of the chromosome number 5 and at interstitial regions of the short arm of chromosome 7. Based on direct visual analysis of chromosome length, morphology and position of FISH signals, a pioneer attempt has been made to construct metaphase karyotype in B. persicum, an endangered medicinal plant of North Western Himalayas.
%K FISH
%K Karyotypes
%K Bunium persicum
%K black cumin
%U http://www.pensoft.net/journals/compcytogen/article/965/